Inbred maize line PH0HR

ABSTRACT

An inbred maize line, designated PH0HR, the plants and seeds of inbred maize line PH0HR, methods for producing a maize plant produced by crossing the inbred line PH0HR with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH0HR with another maize line or plant.

CROSS REFERENCE TO RELATED APPLICATION

This is a continuation in part of copending commonly owned United Statespatent application Inbred Maize Line PH0HR filed Sep. 12, 1995 byWilliam Edward Solawetz (Ser. No. 08/526,810), now abandoned.

FIELD OF THE INVENTION

This invention is in the field of maize breeding, specifically relatingto an inbred maize line designated PH0HR.

BACKGROUND OF THE INVENTION

The goal of plant breeding is to combine in a single variety or hybridvarious desirable traits. For field crops, these traits may includeresistance to diseases and insects, tolerance to heat and drought,reducing the time to crop maturity, greater yield, and better agronomicquality. With mechanical harvesting of many crops, uniformity of plantcharacteristics such as germination and stand establishment, growthrate, maturity, and plant and ear height, is important.

Field crops are bred through techniques that take advantage of theplant's method of pollination. A plant is self-pollinated if pollen fromone flower is transferred to the same or another flower of the sameplant. A plant is cross-pollinated if the pollen comes from a flower ona different plant.

Plants that have been self-pollinated and selected for type for manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny. A cross between twodifferent homozygous lines produces a uniform population of hybridplants that may be heterozygous for many gene loci. A cross of twoplants each heterozygous at a number of gene loci will produce apopulation of hybrid plants that differ genetically and will not beuniform.

Maize (zea mays L.), often referred to as corn in the United States, canbe bred by both self-pollination and cross-pollination techniques. Maizehas separate male and female flowers on the same plant, located on thetassel and the ear, respectively. Natural pollination occurs in maizewhen wind blows pollen from the tassels to the silks that protrude fromthe tops of the ears.

A reliable method of controlling male fertility in plants offers theopportunity for improved plant breeding. This is especially true fordevelopment of maize hybrids, which relies upon some sort of malesterility system. There are several options for controlling malefertility available to breeders, such as: manual or mechanicalemasculation (or detasseling), cytoplasmic male sterility, genetic malesterility, gametocides and the like.

Hybrid maize seed is typically produced by a male sterility systemincorporating manual or mechanical detasseling. Alternate strips of twoinbred varieties of maize are planted in a field, and the pollen-bearingtassels are removed from one of the inbreds (female). Providing thatthere is sufficient isolation from sources of foreign maize pollen, theears of the detasseled inbred will be fertilized only from the otherinbred (male), and the resulting seed is therefore hybrid and will formhybrid plants.

The laborious, and occasionally unreliable, detasseling process can beavoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMSinbred are male sterile as a result of factors resulting from thecytoplasmic, as opposed to the nuclear, genome. Thus, thischaracteristic is inherited exclusively through the female parent inmaize plants, since only the female provides cytoplasm to the fertilizedseed. CMS plants are fertilized with pollen from another inbred that isnot male-sterile. Pollen from the second inbred may or may notcontribute genes that make the hybrid plants male-fertile. Usually seedfrom detasseled fertile maize and CMS produced seed of the same hybridare blended to insure that adequate pollen loads are available forfertilization when the hybrid plants are grown.

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations asdescribed by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. Theseand all patents referred to are incorporated by reference. In additionto these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. patentapplication Ser. No. 07/848,433, have developed a system of nuclear malesterility which includes: identifying a gene which is critical to malefertility; silencing this native gene which is critical to malefertility; removing the native promoter from the essential malefertility gene and replacing it with an inducible promoter; insertingthis genetically engineered gene back into the plant; and thus creatinga plant that is male sterile because the inducible promoter is not "on"resulting in the male fertility gene not being transcribed. Fertility isrestored by inducing, or turning "on", the promoter, which in turnallows the gene that confers male fertility to be transcribed.

There are many other methods of conferring genetic male sterility in theart, each with its own benefits and drawbacks. These methods use avariety of approaches such as delivering into the plant a gene encodinga cytotoxic substance associated with a male tissue specific promoter oran antisense system in which a gene critical to fertility is identifiedand an antisense to that gene is inserted in the plant (see:Fabinjanski, et al. EPO 89/3010153.8 publication no. 329,308 and PCTapplication PCT/CA90/00037 published as WO 90/08828).

Another system useful in controlling male sterility makes use ofgametocides. Gametocides are not a genetic system, but rather a topicalapplication of chemicals. These chemicals affect cells that are criticalto male fertility. The application of these chemicals affects fertilityin the plants only for the growing season in which the gametocide isapplied (see Carlson, Glenn R., U.S. Pat. No.: 4,936,904). Applicationof the gametocide, timing of the application and genotype specificityoften limit the usefulness of the approach.

The use of male sterile inbreds is but one factor in the production ofmaize hybrids. The development of maize hybrids requires, in general,the development of homozygous inbred lines, the crossing of these lines,and the evaluation of the crosses. Pedigree breeding and recurrentselection breeding methods are used to develop inbred lines frombreeding populations. Breeding programs combine the genetic backgroundsfrom two or more inbred lines or various other broad-based sources intobreeding pools from which new inbred lines are developed by selfing andselection of desired phenotypes. The new inbreds are crossed with otherinbred lines and the hybrids from these crosses are evaluated todetermine which of those have commercial potential.

Pedigree breeding starts with the crossing of two genotypes, each ofwhich may have one or more desirable characteristics that is lacking inthe other or which complements the other. If the two original parents donot provide all the desired characteristics, other sources can beincluded in the breeding population. In the pedigree method, superiorplants are selfed and selected in successive generations. In thesucceeding generations the heterozygous condition gives way tohomogeneous lines as a result of self-pollination and selection.Typically in the pedigree method of breeding five or more generations ofselfing and selection is practiced: F₁ →F₂ ; F₃ →F₄ ; F₄ →F₅, etc.

Recurrent selection breeding, backcrossing for example, can be used toimprove an inbred line. Backcrossing can be used to transfer a specificdesirable trait from one inbred or source to an inbred that lacks thattrait. This can be accomplished, for example, by first crossing asuperior inbred (recurrent parent) to a donor inbred (non-recurrentparent), that carries the appropriate gene(s) for the trait in question.The progeny of this cross is then mated back to the superior recurrentparent followed by selection in the resultant progeny for the desiredtrait to be transferred from the non-recurrent parent. After five ormore backcross generations with selection for the desired trait, theprogeny will be heterozygous for loci controlling the characteristicbeing transferred, but will be like the superior parent for most oralmost all other genes. The last backcross generation is then selfed togive pure breeding progeny for the gene(s) being transferred.

A single cross hybrid maize variety is the cross of two inbred lines,each of which has a genotype that complements the genotype of the other.The hybrid progeny of the first generation is designated F₁. In thedevelopment of hybrids only the F₁ hybrid plants are sought. PreferredF₁ hybrids are more vigorous than their inbred parents. This hybridvigor, or heterosis, can be manifested in many polygenic traits,including increased vegetative growth and increased yield.

The development of a hybrid maize variety involves three steps: (1) theselection of plants from various germplasm pools for initial breedingcrosses; (2) the selfing of the selected plants from the breedingcrosses for several generations to produce a series of inbred lines,which, although different from each other, breed true and are highlyuniform; and (3) crossing the selected inbred lines with differentinbred lines to produce the hybrid progeny (F₁). During the inbreedingprocess in maize, the vigor of the lines decreases. Vigor is restoredwhen two different inbred lines are crossed to produce the hybridprogeny (F₁). An important consequence of the homozygosity andhomogeneity of the inbred lines is that the hybrid between a definedpair of inbreds will always be the same. Once the inbreds that give asuperior hybrid have been identified, the hybrid seed can be reproducedindefinitely as long as the homogeneity of the inbred parents ismaintained.

A single cross hybrid is produced when two inbred lines are crossed toproduce the F₁ progeny. A double cross hybrid is produced from fourinbred lines crossed in pairs (A×B and C×D) and then the two F₁ hybridsare crossed again (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁hybrids is lost in the next generation (F₂). Consequently, seed fromhybrid varieties is not used for planting stock.

Maize is an important and valuable field crop. Thus, a continuing goalof plant breeders is to develop high-yielding maize hybrids that areagronomically sound based on stable inbred lines. The reasons for thisgoal are obvious: to maximize the amount of grain produced with theinputs used and minimize susceptibility of the crop to environmentalstresses. To accomplish this goal, the maize breeder must select anddevelop superior inbred parental lines for producing hybrids. Thisrequires identification and selection of genetically unique individualsthat occur in a segregating population. The segregating population isthe result of a combination of crossover events plus the independentassortment of specific combinations of alleles at many gene loci thatresults in specific genotypes. Based on the number of segregating genes,the frequency of occurrence of an individual with a specific genotype isless than 1 in 10,000. Thus, even if the entire genotype of the parentshas been characterized and the desired genotype is known, only a few ifany individuals having the desired genotype may be found in a large F₂or S₀ population. Typically, however, the genotype of neither theparents nor the desired genotype is known in any detail.

In addition to the preceding problem, it is not known how the genotypewill react with the environment. This genotype by environmentinteraction is an important, yet unpredictable, factor in plantbreeding. A breeder of ordinary skill in the art cannot predict thegenotype, how that genotype will interact with various environments orthe resulting phenotypes of the developing lines, except perhaps in avery broad and general fashion. A breeder of ordinary skill in the artwould also be unable to recreate the same line twice from the very sameoriginal parents as the breeder is unable to direct how the genomescombine or how they will interact with the environmental conditions.This unpredictability results in the expenditure of large amounts ofresearch resources in the development of a superior new maize inbredline.

Pioneer research station staff propose about 400 to 500 new inbreds eachyear from over 2,000,000 pollinations. Of those proposed new inbreds,less than 50 and more commonly less than 30 are actually selected forcommercial use.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred maize line,designated PH0HR. This invention thus relates to the seeds of inbredmaize line PH0HR, to the plants of inbred maize line PH0HR, and tomethods for producing a maize plant produced by crossing the inbred linePH0HR with itself or another maize line. This invention further relatesto hybrid maize seeds and plants produced by crossing the inbred linePH0HR with another maize line.

DEFINITIONS

In the description and examples that follow, a number of terms are usedherein. In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided. NOTE: ABS is in absolute termsand %MN is percent of the mean for the experiments in which the inbredor hybrid was grown. These designators will follow the descriptors todenote how the values are to be interpreted. Below are the descriptorsused in the data tables included herein.

ANT ROT=ANTHRACNOSE STALK ROT (Colletotrichum graminicola). A 1 to 9visual rating indicating the resistance to Anthracnose Stalk Rot. Ahigher score indicates a higher resistance.

BAR PLT=BARREN PLANTS. The percent of plants per plot that were notbarren (lack ears).

BRT STK=BRITTLE STALKS. This is a measure of the stalk breakage near thetime of pollination, and is an indication of whether a hybrid or inbredwould snap or break near the time of flowering under severe winds. Dataare presented as percentage of plants that did not snap.

BU ACR=YIELD (BUSHELS/ACRE). Yield of the grain at harvest in bushelsper acre adjusted to 15.5% moisture.

CLN=CORN LETHAL NECROSIS. Synergistic interaction of maize chloroticmottle virus (MCMV) in combination with either maize dwarf mosaic virus(MDMV-A or MDMV-B) or wheat streak mosaic virus (WSMV). A 1 to 9 visualrating indicating the resistance to Corn Lethal Necrosis. A higher scoreindicates a higher resistance.

COM RST=COMMON RUST (Puccinia sorghi). A 1 to 9 visual rating indicatingthe resistance to Common Rust. A higher score indicates a higherresistance.

D/D=DRYDOWN. This represents the relative rate at which a hybrid willreach acceptable harvest moisture compared to other hybrids on a 1-9rating scale. A high score indicates a hybrid that dries relatively fastwhile a low score indicates a hybrid that dries slowly.

DIP ERS=DIPLODIA EAR MOLD SCORES (Diplodia maydis and Diplodiamacrospora). A 1 to 9 visual rating indicating the resistance toDiplodia Ear Mold. A higher score indicates a higher resistance.

DRP EAR=DROPPED EARS. A measure of the number of dropped ears per plotand represents the percentage of plants that did not drop ears prior toharvest.

D/T=DROUGHT TOLERANCE. This represents a 1-9 rating for droughttolerance, and is based on data obtained under stress conditions. A highscore indicates good drought tolerance and a low score indicates poordrought tolerance.

EAR HT=EAR HEIGHT. The ear height is a measure from the ground to thehighest placed developed ear node attachment and is measured in inches.

EAR MLD=General Ear Mold. Visual rating (1-9 score) where a "1" is verysusceptible and a "9" is very resistant. This is based on overall ratingfor ear mold of mature ears without determining the specific moldorganism, and may not be predictive for a specific ear mold.

EAR SZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher therating the larger the ear size.

ECB 1LF=EUROPEAN CORN BORER FIRST GENERATION LEAF FEEDING (Ostrinianubilalis). A 1 to 9 visual rating indicating the resistance topreflowering leaf feeding by first generation European Corn Borer. Ahigher score indicates a higher resistance.

ECB 2IT=EUROPEAN CORN BORER SECOND GENERATION INCHES OF TUNNELING(Ostrinia nubilalis). Average inches of tunneling per plant in thestalk.

ECB 2SC=EUROPEAN CORN BORER SECOND GENERATION (Ostrinia nubilalis). A 1to 9 visual rating indicating post flowering degree of stalk breakageand other evidence of feeding by European Corn Borer, Second Generation.A higher score indicates a higher resistance.

ECB DPE=EUROPEAN CORN BORER DROPPED EARS (Ostrinia nubilalis). Droppedears due to European Corn Borer. Percentage of plants that did not dropears under second generation corn borer infestation.

EST CNT=EARLY STAND COUNT. This is a measure of the stand establishmentin the spring and represents the number of plants that emerge on perplot basis for the inbred or hybrid.

EYE SPT=Eye Spot (Kabatiella zeae or Aureobasidium zeae). A 1 to 9visual rating indicating the resistance to Eye Spot. A higher scoreindicates a higher resistance.

FUS ERS=FUSARIUM EAR ROT SCORE (Fusarium moniliforme or Fusariumsubglutinans). A 1 to 9 visual rating indicating the resistance toFusarium ear rot. A higher score indicates a higher resistance.

GDU=Growing Degree Units. Using the Barger Heat Unit Theory, whichassumes that maize growth occurs in the temperature range 50° F.-86° F.and that temperatures outside this range slow down growth; the maximumdaily heat unit accumulation is 36 and the minimum daily heat unitaccumulation is 0. The seasonal accumulation of GDU is a major factor indetermining maturity zones.

GDU SHD=GDU TO SHED. The number of growing degree units (GDUs) or heatunits required for an inbred line or hybrid to have approximately 50percent of the plants shedding pollen and is measured from the time ofplanting. Growing degree units are calculated by the Barger Method,where the heat units for a 24-hour period are: ##EQU1##

The highest maximum temperature used is 86° F. and the lowest minimumtemperature used is 50° F. For each inbred or hybrid it takes a certainnumber of GDUs to reach various stages of plant development.

GDU SLK=GDU TO SILK. The number of growing degree units required for aninbred line or hybrid to have approximately 50 percent of the plantswith silk emergence from time of planting. Growing degree units arecalculated by the Barger Method as given in GDU SHD definition.

GIB ERS=GIBBERELLA EAR ROT (PINK MOLD) (Gibberella zeae). A 1 to 9visual rating indicating the resistance to Gibberella Ear Rot. A higherscore indicates a higher resistance.

GLF SPT=Gray Leaf Spot (Cercospora zeae-maydis). A 1 to 9 visual ratingindicating the resistance to Gray Leaf Spot. A higher scone indicates ahigher resistance.

GOS WLT=Goss' Wilt (Corynebacterium nebraskense). A 1 to 9 visual ratingindicating the resistance to Goss' Wilt. A higher score indicates ahigher resistance.

GRN APP=GRAIN APPEARANCE. This is a 1 to 9 rating for the generalappearance of the shelled grain as it is harvested based on such factorsas the color of harvested grain, any mold on the grain, and any crackedgrain. High scores indicate good grain quality.

H/POP=YIELD AT HIGH DENSITY. Yield ability at relatively high plantdensities on 1-9 relative rating system with a higher number indicatingthe hybrid responds well to high plant densities for yield relative toother hybrids. A 1, 5, and 9 would represent very poor, average, andvery good yield response, respectively, to increased plant density.

HC BLT=HELMINTHOSPORIUM CARBONUM LEAF BLIGHT (Helminthosporiumcarbonum). A 1 to 9 visual rating indicating the resistance toHelminthosporium infection. A higher score indicates a higherresistance.

HD SMT=HEAD SMUT (Sphacelotheca reiliana). This score indicates thepercentage of plants not infected.

INC D/A=GROSS INCOME (DOLLARS PER ACRE). Relative income per acreassuming drying costs of two cents per point above 15.5 percent harvestmoisture and current market price per bushel.

INCOME/ACRE. Income advantage of hybrid to be patented over other hybridon per acre basis.

INC ADV=GROSS INCOME ADVANTAGE. GROSS INCOME advantage of variety #1over variety #2.

L/POP=YIELD AT LOW DENSITY. Yield ability at relatively low plantdensities on a 1-9 relative system with a higher number indicating thehybrid responds well to low plant densities for yield relative to otherhybrids. A 1, 5, and 9 would represent very poor, average, and very goodyield response, respectively, to low plant density.

MDM CPX=MAIZE DWARF MOSAIC COMPLEX (MDMV=Maize Dwarf Mosaic Virus andMCDV=Maize Chlorotic Dwarf Virus). A 1 to 9 visual rating indicating theresistance to Maize Dwarf Mosaic Complex. A higher score indicates ahigher resistance.

MST=HARVEST MOISTURE. The moisture is the actual percentage moisture ofthe grain at harvest.

MST ADV=MOISTURE ADVANTAGE. The moisture advantage of variety #1 overvariety #2 as calculated by: MOISTURE of variety #2-MOISTURE of variety#1=MOISTURE ADVANTAGE of variety #1.

NLF BLT=Northern Leaf Blight (Helminthosporium turcicum or Exserohilumturcicum). A 1 to 9 visual rating indicating the resistance to NorthernLeaf Blight. A higher score indicates a higher resistance.

PLT HT=PLANT HEIGHT. This is a measure of the height of the plant fromthe ground to the tip of the tassel in inches.

POL SC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount ofpollen shed. The higher the score the more pollen shed.

POL WT=POLLEN WEIGHT. This is calculated by dry weight of tasselscollected as shedding commences minus dry weight from similar tasselsharvested after shedding is complete.

It should be understood that the inbred can, through routinemanipulation of cytoplasmic or other factors, be produced in amale-sterile form. Such embodiments are also contemplated within thescope of the present claims.

POP K/A=PLANT POPULATIONS. Measured as 1000s per acre.

POP ADV=PLANT POPULATION ADVANTAGE. The plant population advantage ofvariety #1 over variety #2 as calculated by PLANT POPULATION of variety#2-PLANT POPULATION of variety #1=PLANT POPULATION ADVANTAGE of variety#1.

PRM=PREDICTED RELATIVE MATURITY. This trait, predicted relativematurity, is based on the harvest moisture of the grain. The relativematurity rating is based on a known set of checks and utilizes standardlinear regression analyses and is also referred to as the ComparativeRelative Maturity Rating System that is similar to the MinnesotaRelative Maturity Rating System.

PRM SHD=A relative measure of the growing degree units (GDU) required toreach 50% pollen shed. Relative values are predicted values from thelinear regression of observed GDU's on relative maturity of commercialchecks.

RT LDG=ROOT LODGING. Root lodging is the percentage of plants that donot root lodge; plants that lean from the vertical axis at anapproximately 30° angle or greater would be counted as root lodged.

RTL ADV=ROOT LODGING ADVANTAGE. The root lodging advantage of variety #1over variety #2.

SCT GRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount ofscatter grain (lack of pollination or kernel abortion) on the ear. Thehigher the score the less scatter grain.

SDG VGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amountof vegetative growth after emergence at the seedling stage(approximately five leaves). A higher score indicates better vigor.

SEL IND=SELECTION INDEX. The selection index gives a single measure ofthe hybrid's worth based on information for up to five traits. A maizebreeder may utilize his or her own set of traits for the selectionindex. One of the traits that is almost always included is yield. Theselection index data presented in the tables represent the mean valueaveraged across testing stations.

SLF BLT=SOUTHERN LEAF BLIGHT (Helminthosporium maydis or Bipolarismaydis). A 1 to 9 visual rating indicating the resistance to SouthernLeaf Blight. A higher score indicates a higher resistance.

SOU RST=SOUTHERN RUST (Puccinia polysora). A 1 to 9 visual ratingindicating the resistance to Southern Rust. A higher score indicates ahigher resistance.

STA GRN=STAY GREEN. Stay green is the measure of plant health near thetime of black layer formation (physiological maturity). A high scoreindicates better late-season plant health.

STD ADV=STALK STANDING ADVANTAGE. The advantage of variety #1 overvariety #2 for the trait STK CNT.

STK CNT=NUMBER OF PLANTS. This is the final stand or number of plantsper plot.

STK LDG=STALK LODGING. This is the percentage of plants that did notstalk lodge (stalk breakage) as measured by either natural lodging orpushing the stalks and determining the percentage of plants that breakbelow the ear.

STW WLT=Stewart's Wilt (Erwinia stewartii). A 1 to 9 visual ratingindicating the resistance to Stewart's Wilt. A higher score indicates ahigher resistance.

TAS BLS=TASSEL BLAST. A 1 to 9 visual rating was used to measure thedegree of blasting (necrosis due to heat stress) of the tassel at thetime of flowering. A 1 would indicate a very high level of blasting attime of flowering, while a 9 would have no tassel blasting.

TAS SZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate therelative size of the tassel. The higher the rating the larger thetassel.

TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams)just prior to pollen shed.

TEX EAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate therelative hardness (smoothness of crown) of mature grain. A 1 would bevery soft (extreme dent) while a 9 would be very hard (flinty or verysmooth crown).

TILLER=TILLERS. A count of the number of tillers per plot that couldpossibly shed pollen was taken. Data is given as a percentage oftillers: number of tillers per plot divided by number of plants perplot.

TST WT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grainin pounds for a given volume (bushel).

TST WTA=TEST WEIGHT ADJUSTED. The measure of the weight of the grain inpounds for a given volume (bushel) adjusted for 15.5 percent moisture.

TSW ADV=TEST WEIGHT ADVANTAGE. The test weight advantage of variety #1over variety #2.

WIN M %=PERCENT MOISTURE WINS.

WIN Y %=PERCENT YIELD WINS.

YLD=YIELD. It is the same as BU ACR ABS.

YLD ADV=YIELD ADVANTAGE. The yield advantage of variety #1 over variety#2 as calculated by: YIELD of variety #1-YIELD variety #2=yieldadvantage of variety #1.

YLD SC=YIELD SCORE. A 1 to 9 visual rating was used to give a relativerating for yield based on plot ear piles. The higher the rating thegreater visual yield appearance.

DETAILED DESCRIPTION OF THE INVENTION

Inbred maize lines are typically developed for use in the production ofhybrid maize lines. Inbred maize lines need to be highly homogeneous,homozygous and reproducible to be useful as parents of commercialhybrids. There are many analytical methods available to determine thehomozygotic and phenotypic stability of these inbred lines.

The oldest and most traditional method of analysis is the observation ofphenotypic traits. The data is usually collected in field experimentsover the life of the maize plants to be examined. Phenotypiccharacteristics most often observed are for traits associated with plantmorphology, ear and kernel morphology, insect and disease resistance,maturity, and yield.

In addition to phenotypic observations, the genotype of a plant can alsobe examined. There are many laboratory-based techniques available forthe analysis, comparison and characterization of plant genotype; amongthese are Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length Polymorphisms (AFLPs), and Simple SequenceRepeats (SSRs) which are also referred to as Microsatellites.

The most widely used of these laboratory techniques are IsozymeElectrophoresis and RFLPs as discussed in Lee, M., "Inbred Lines ofMaize and Their Molecular Markers," The Maize Handbook,(Springer-Verlag, New York, Inc. 1994, at 423-432) incorporated hereinby reference. Isozyme Electrophoresis is a useful tool in determininggenetic composition, although it has relatively low number of availablemarkers and the low number of allelic variants among maize inbreds.RFLPs have the advantage of revealing an exceptionally high degree ofallelic variation in maize and the number of available markers is almostlimitless.

Maize RFLP linkage maps have been rapidly constructed and widelyimplemented in genetic studies. One such study is described inBoppenmaier, et al., "Comparisons among strains of inbreds for RFLPs",Maize Genetics Cooperative Newsletter, 65:1991, pg. 90, is incorporatedherein by reference. This study used 101 RFLP markers to analyze thepatterns of 2 to 3 different deposits each of five different inbredlines. The inbred lines had been selfed from 9 to 12 times before beingadopted into 2 to 3 different breeding programs. It was results fromthese 2 to 3 different breeding programs that supplied the differentdeposits for analysis. These five lines were maintained in the separatebreeding programs by selfing or sibbing and rogueing off-type plants foran additional one to eight generations. After the RFLP analysis wascompleted, it was determined the five lines showed 0-2% residualheterozygosity. Although this was a relatively small study, it can beseen using RFLPs that the lines had been highly homozygous prior to theseparate strain maintenance.

Inbred maize line PH0HR is a yellow, dent maize inbred that is bestsuited as either a male or a female in crosses for producing firstgeneration F₁ maize hybrids. Inbred maize line PH0HR is best adapted tothe Northcentral and Northeast region of the United States and farSouthern Ontario, Canada, and can be used to produce hybrids from anaverage relative maturity of 104 based on the Comparative RelativeMaturity Rating System for harvest moisture of grain. Inbred maize linePH0HR is high yielding as an inbred per se. In hybrid combination, PH0HRis high yielding for its maturity, exhibits late season plant health andhas short plant stature.

The inbred has shown uniformity and stability within the limits ofenvironmental influence for all the traits as described in the VarietyDescription Information (Table 1) that follows. The inbred has beenself-pollinated and ear-rowed a sufficient number of generations withcareful attention paid to uniformity of plant type to ensure thehomozygosity and phenotypic stability necessary to use in commercialproduction. The line has been increased both by hand and in isolatedfields with continued observation for uniformity. No variant traits havebeen observed or are expected in PH0HR.

Inbred maize line PH0HR, being substantially hornozygous, can bereproduced by planting seeds of the line, growing the resulting maizeplants under self-pollinating or sib-pollinating conditions withadequate isolation, and harvesting the resulting seed, using techniquesfamiliar to the agricultural arts.

                  TABLE 1    ______________________________________    VARIETY DESCRIPTION INFORMATION    INBRED = PH0HR    ______________________________________        Type: Dent    A.  Maturity: Average cross maturity zones. Zone: 0        GDU Shed: 1400        GDU Silk: 1400        No. Reps: 62    B.  Tassel:        Anther Color: Yellow*        Glume Color: Green*    C.  Ear (Husked Ear Data Except When Stated Otherwise):        Silk Color: Yellow*    D.  Cob:        Color: Red*    E.  Diseases:        Eye Spot (K. zeae): Resistant        Gray Leaf Spot (C. zeae): Susceptible    F.  Insects:        European Corn Borer-1 Leaf Damage (Pre-flowering): Resistant        European Corn Borer-2 (Post-flowering): Intermediate        The above descriptions are based on a scale of 1-9, 1 being highly        susceptible, 9 being highly resistant.        S (Susceptible): Would generally represent a score of 1-3.        I (Intermediate): Would generally represent a score of 4-5.        R (Resistant): Would generally represent a score of 6-7.        H (Highly Resistant) Would generally represent a score of 8-9.        Highly resistant does not imply the inbred is immune.    ______________________________________     *In interpreting the foregoing color designations, reference may be had t     the Munsell Glossy Book of Color, a standard color reference.     Data for Items B, C, D, E, F, and G is based primarily on description     information from the maintaining station.

Industrial Applicability

This invention also is directed to methods for producing a maize plantby crossing a first parent maize plant with a second parent maize plantwherein the first or second parent maize plant is an inbred maize plantof the line PH0HR. Further, both first and second parent maize plantscan come from the inbred maize line PH0HR. Thus, any such methods usingthe inbred maize line PH0HR are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, and the like.All plants produced using inbred maize line PH0HR as a parent are withinthe scope of this invention. Advantageously, the inbred maize line isused in crosses with other, different, maize inbreds to produce firstgeneration (F₁) maize hybrid seeds and plants with superiorcharacteristics.

As used herein, the term plant includes plant cells, plant protoplasts,plant cell tissue cultures from which maize plants can be regenerated,plant calli, plant clumps, and plant cells that are intact in plants orparts of plants, such as embryos, pollen, ovules, flowers, kernels,ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk andthe like.

Duncan, Williams, Zehr, and Widholm, Planta (1985) 165:322-332 reflectsthat 97% of the plants cultured that produced callus were capable ofplant regeneration. Subsequent experiments with both inbreds and hybridsproduced 91% regenerable callus that produced plants. In a further studyin 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988),7:262-265 reports several media additions that enhance regenerability ofcallus of two inbred lines. Other published reports also indicated that"nontraditional" tissues are capable of producing somatic embryogenesisand plant regeneration. K. P. Rao, et al., Maize Genetics CooperationNewsletter, 60:64-65 (1986), refers to somatic embryogenesis from glumecallus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345-347(1987) indicates somatic embryogenesis from the tissue cultures of maizeleaf segments. Thus, it is clear from the literature that the state ofthe art is such that these methods of obtaining plants are, and were,"conventional" in the sense that they are routinely used and have a veryhigh rate of success.

Tissue culture of maize is described in European Patent Application,publication 160,390, incorporated herein by reference. Maize tissueculture procedures are also described in Green and Rhodes, "PlantRegeneration in Tissue Culture of Maize," Maize for Biological Research(Plant Molecular Biology Association, Charlottesville, Va. 1982, at367-372) and in Duncan, et al., "The Production of Callus Capable ofPlant Regeneration from Immature Embryos of Numerous Zea MaysGenotypes," 165 Planta 322-332 (1985). Thus, another aspect of thisinvention is to provide cells which upon growth and differentiationproduce maize plants having the physiological and morphologicalcharacteristics of inbred line PH0HR.

Maize is used as human food, livestock feed, and as raw material inindustry. The food uses of maize, in addition to human consumption ofmaize kernels, include both products of dry- and wet-milling industries.The principal products of maize dry milling are grits, meal and flour.The maize wet-milling industry can provide maize starch, maize syrups,and dextrose for food use. Maize oil is recovered from maize germ, whichis a by-product of both dry- and wet-milling industries.

Maize, including both grain and non-grain portions of the plant, is alsoused extensively as livestock feed, primarily for beef cattle, dairycattle, hogs, and poultry.

Industrial uses of maize include production of ethanol, maize starch inthe wet-milling industry and maize flour in the dry-milling industry.The industrial applications of maize starch and flour are based onfunctional properties, such as viscosity, film formation, adhesiveproperties, and ability to suspend particles. The maize starch and flourhave application in the paper and textile industries. Other industrialuses include applications in adhesives, building materials, foundrybinders, laundry starches, explosives, oil-well muds, and other miningapplications.

Plant parts other than the grain of maize are also used in industry: forexample, stalks and husks are made into paper and wallboard and cobs areused for fuel and to make charcoal.

The seed of inbred maize line PH0HR, the plant produced from the inbredseed, the hybrid maize plant produced from the crossing of the inbred,hybrid seed, and various parts of the hybrid maize plant can be utilizedfor human food, livestock feed, and as a raw material in industry.

PERFORMANCE EXAMPLES OF PH0HR

In the examples that follow, the traits and characteristics of inbredmaize line PH0HR are presented as an inbred per se and in hybridcombination. The data collected on inbred maize line PH0HR is presentedfor key characteristics and traits.

The results in Table 2A compare inbred PH0HR to inbred PHBW8 althoughinbred PH0HR is lower yielding than PHBW8, inbred PH0HR demonstrates alower harvest moisture than inbred PHBW8. The inbred per se results showthat PH0HR has significantly shorter stature than PHBW8.

The results in Table 2B compare PH0HR to one of its parents, PHP02. Theinbred per se results show both inbreds have above average yield,although PHP02 is higher yielding. Inbred PH0HR has significantly higherpollen weight and higher pollen score than PHP02. In addition inbredPH0HR is significantly shorter in stature than PHP02.

Table 2C compares PH0HR to another line with similar maturity, PH24E.The inbred per se results show PH0HR has significantly higher seedlingvigor and higher early stand count than PH24E.

The results in Table 3A compare the general combining of PH0HR andPHP02. The results show the PH0HR hybrids are significantly higheryielding, have similar test weight and have higher seedling vigor thanthe PHP02 hybrids. In addition, the PH0HR hybrids have a significantlyhigher stay green score than the PHP02 hybrids and are significantlymore resistant to brittle snap.

Table 3B compares the general combining ability of PH0HR and PH24E. Theresults show that both hybrids are high yielding with PH0HR hybridsdemonstrating significantly higher seedling vigor than the PH24Ehybrids. The PH0HR hybrids have a significantly higher stay green scoreand significantly higher resistance to brittle snap than the PH24Ehybrids.

The results in Table 4A compare the specific combining ability of PH0HRand PHRD6 when crossed to PHRE1 and PHP02. These two hybrids are ofequal early maturity. The results show that both hybrids are highyielding. The PH0HR hybrid is shorter with significantly higher earplacement than the PHRD6 hybrid. The PH0HR also has a significantlyhigher seedling vigor score and a higher stay green score than the PHP02hybrid.

The results in Table 4B compare the specific combining ability of PH0HRand PHP02 when crossed to PHRE1. The results show the PH0HR hybrid issignificantly higher yielding than the PHP02 hybrid. In addition, thePH0HR hybrid has significantly superior stay green and resistance tobrittle snap. Both hybrids are below average in plant stature, andexhibits above average seedling vigor.

The results in Table 4C compare the specific combining ability of PH0HRand PHTM9 crossed with PHRE1 and PHP02. The PHTM9 hybrid is a widelygrown hybrid of earlier maturity than the PH0HR hybrid. The results showthat the PH0HR hybrid is significantly higher yielding and shorter instature with a significantly superior stay green score than the PHTM9hybrid. Both hybrids had above average grain appearance and seedlingvigor scores.

The results in Table 4D demonstrate the specific combining ability ofPH0HR and PHTD5 crossed to PHRE1. As can be seen from the table, thePH0HR hybrid is significantly higher yielding and is significantlytaller than the PHTD5 hybrid. The PH0HR hybrid also has a superior staygreen score. Both hybrids exhibit above average seedling vigor.

The results in Table 4E compare the specific combining ability of PH0HRand PH0AV crossed to PHRE1 and PHO02. The PH0AV hybrid is slightlyearlier and is representative of genetics that have historically donevery well in the maturity. The results show that the PH0HR hybrid issignificantly higher yielding with a significantly higher seedling vigorscore than the PH0AV hybrid. The PH0HR hybrid also has significantlyshorter stature and excellent stay green with a significantly higherstay green score than the PH0AV hybrid.

The results in Table 4F compare PH0HR and PH24E crossed to PHRE1 andPH0HC to show the specific combining ability of the inbreds. PHRE1 andPH0HC. The PH0HC hybrid is high yielding and slighter later in maturitythan the PH0HR hybrid. The results show that both hybrids are highyielding with the PH0HR hybrid demonstrating significantly lower harvestmoisture than the PH24E hybrid. The PH0HR hybrid also has asignificantly higher early stand count, and higher resistance to brittlestalk than the PH24E hybrid. Both hybrids exhibit above average seedlingvigor and stay green scores.

The results in Table 4G compare the specific combining ability of PH0HRand PH24E crossed to PHRE1 and PH38D. The PH24E is a high yieldinghybrid approximately four days later in maturity than the PH0HR hybrid.The results show that the PH0HR hybrid has significantly lower harvestmoisture and an excellent seedling vigor with a very significantlyhigher seedling vigor score than the PH24E hybrid. The PH0HR hybrid isalso significantly shorter than the PH24E hybrid.

The results in Table 4H compare the specific combining ability of PH0HRand PH24E crossed to PHRE1 and PH44A. The PH24E hybrid is considerablylater in maturity than the PH0HR hybrid. The results demonstrate thatboth hybrids are high yielding and the PH0HR hybrid has significantlylower harvest moisture than the PH24E hybrid. The PH0HR hybrid has asignificantly shorter plant stature, higher seedling vigor score andsuperior resistance to brittle snap.

                                      TABLE 2A    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT    VARIETY #1 = PH0HR    VARIETY #2 = PHBW8    __________________________________________________________________________               BU BU     TST                            SDG                               EST                                  TIL                                     GDU                                        GDU               ACR                  ACR MST                         WT VGR                               CNT                                  LER                                     SHD                                        SLK               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   50.1                  102 15.8                         58.2                            4.2                               37.0                                  4.4                                     135.7                                        139.5           2   72.6                  149 17.4                         57.9                            4.9                               33.6                                  2.7                                     134.1                                        135.5           LOCS               3  3   3  3  37 60 35 49 49           REPS               5  5   5  5  43 68 39 51 51           DIFF               22.4                  48  1.6                         0.2                            0.7                               3.4                                  1.8                                     1.6                                        3.9           PR > T               .030+                  .047+                      .360                         .751                            .005#                               .005*                                  .098*                                     .006#                                        .000#    __________________________________________________________________________               POL                  TAS PLT                         EAR                            RT STA                                  STK                                     BRT                                        GRN               SC SZ  HT HT LDG                               GRN                                  LDG                                     STK                                        APP               ABS                  ABS ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   7.0                  6.2 68.9                         28.4                            98.2                               3.9                                  93.8                                     100.0                                        7.8           2   4.3                  4.4 77.0                         30.4                            82.0                               4.8                                  97.0                                     96.4                                        5.7           LOCS               6  15  14 11 7  11 4  4  3           REPS               6  15  16 13 7  13 6  4  5           DIFF               2.7                  1.8 8.1                         2.0                            16.2                               0.9                                  3.3                                     3.6                                        2.2           PR > T               .007#                  .000#                      .000#                         .307                            .082*                               .138                                  .406                                     .391                                        .069*    __________________________________________________________________________               SCT                  EAR TEX                         EAR                            BAR                               DRP                                  GLF                                     NLF                                        STW               GRN                  SZ  EAR                         MLD                            PLT                               EAR                                  SPT                                     BLT                                        WLT               ABS                  ABS ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   7.2                  5.6 3.8                         6.0                            98.5                               99.3                                  5.0                                     6.0                                        2.0           2   7.6                  6.7 6.3                         6.6                            98.0                               100.0                                  4.0                                     7.0                                        6.0           LOCS               5  7   4  5  11 3  1  1  2           REPS               5  7   4  5  11 5  1  1  2           DIFF               0.4                  1.1 2.5                         0.6                            0.5                               0.7                                  1.0                                     1.0                                        4.0           PR > T               .178                  .000#                      .030+                         .426                            .481                               .423     .000#    __________________________________________________________________________               EYE                  ECB ECB               SPT                  1LF 2SC               ABS                  ABS ABS    __________________________________________________________________________    TOTAL SUM           1   7.0                  6.0 4.5           2   7.0                  3.8 6.5           LOCS               1  6   2           REPS               1  6   2           DIFF               0.0                  2.2 2.0           PR > T .063*                      .295    __________________________________________________________________________     * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 2B    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT    VARIETY #1 = PH0HR    VARIETY #2 = PHP02    __________________________________________________________________________               BU BU     TST                            SDG                               EST                                  TIL                                     GDU                                        GDU               ACR                  ACR MST                         WT VGR                               CNT                                  LER                                     SHD                                        SLK               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   81.2                  111 23.5                         53.3                            4.4                               35.3                                  4.9                                     134.5                                        137.8           2   91.0                  125 21.4                         53.4                            4.5                               33.9                                  3.9                                     129.7                                        132.7           LOCS               28 28  33 24 59 113                                  53 88 84           REPS               44 44  49 38 67 154                                  64 95 89           DIFF               9.9                  14  2.1                         0.1                            0.1                               1.3                                  1.1                                     4.8                                        5.1           PR > T               .000#                  .000#                      .000#                         .575                            .551                               .087*                                  .153                                     .000#                                        .000#    __________________________________________________________________________               POL                  POL POL                         TAS                            TAS                               PLT                                  EAR                                     RT STA               WT WT  SC BLS                            SZ HT HT LDG                                        GRN               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   114.8                  94  6.1                         9.0                            6.1                               68.6                                  28.9                                     96.9                                        4.3           2   82.5                  69  5.9                         9.0                            5.9                               73.3                                  28.7                                     96.6                                        3.2           LOCS               6  6   14 1  38 33 27 15 12           REPS               6  6   15 1  40 40 33 18 14           DIFF               32.3                  26  0.3                         0.0                            0.1                               4.7                                  0.2                                     0.3                                        1.2           PR > T               .009#                  .008#                      .405  .473                               .000#                                  .835                                     .880                                        .048+    __________________________________________________________________________               STK                  BRT GRN                         SCT                            EAR                               TEX                                  EAR                                     BAR                                        DRP               LDG                  STK APP                         GPN                            SZ EAR                                  MLD                                     PLT                                        EAR               ABS                  ABS ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   97.5                  100.0                      6.1                         7.9                            5.7                               3.5                                  5.9                                     98.0                                        99.7           2   95.1                  98.0                      6.3                         7.6                            6.3                               3.4                                  6.6                                     98.8                                        98.9           LOCS               17 9   11 8  7  7  10 39 14           REPS               26 9   18 9  7  8  11 41 21           DIFF               2.4                  2.0 0.2                         0.3                            0.6                               0.1                                  0.7                                     0.8                                        0.8           PR > T               .347                  .118                      .658                         .451                            .103                               .604                                  .045+                                     .133                                        .250    __________________________________________________________________________               GLF                  NLF GOS                         STW                            HD    GIB                                     EYE                                        COM               SPT                  BLT WLT                         WLT                            SMT                               CLN                                  ERS                                     SPT                                        RST               ABS                  ABS ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   5.3                  4.8 6.8                         2.8                            55.5                               2.5                                  4.0                                     7.0                                        5.2           2   4.3                  4.3 5.0                         2.5                            94.4                               4.5                                  5.5                                     6.0                                        6.0           LOCS               4  6   2  3  2  1  1  1  3           REPS               6  9   3  4  4  2  2  1  5           DIFF               1.0                  0.4 1.8                         0.3                            38.9                               2.0                                  1.5                                     1.0                                        0.8           PR > T               .016+                  .317                      .395                         .423                            .324        .199    __________________________________________________________________________               ECB                  ECB               1LF                  2SC               ABS                  ABS    __________________________________________________________________________    TOTAL SUM           1   5.8                  5.3           2   5.3                  5.2           LOCS               8  3           REPS               8  4           DIFF               0.5                  0.2           PR > T               .381                  .423    __________________________________________________________________________     * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 2C    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT    VARIETY #1 = PH0HR    VARIETY #2 = PH24E    __________________________________________________________________________               BU BU     TST                            SDG                               EST                                  TIL                                     GDU                                        GDU               ACR                  ACR MST                         WT VGR                               CNT                                  LER                                     SHD                                        SLK               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   68.2                  106 22.7                         54.1                            4.5                               33.0                                  5.2                                     135.6                                        139.4           2   85.5                  134 21.7                         54.4                            3.6                               32.1                                  3.6                                     138.0                                        141.9           LOCS               19 19  19 15 62 102                                  53 91 89           REPS               26 26  26 20 68 133                                  59 96 94           DIFF               17.3                  28  1.0                         0.3                            1.0                               0.9                                  1.6                                     2.4                                        2.5           PR > T               .001#                  .002#                      .101                         .513                            .000#                               .125                                  .375                                     .000#                                        .000#    __________________________________________________________________________               POL                  POL POL                         TAS                            TAS                               PLT                                  EAR                                     RT STA               WT WT  SC BLS                            SZ HT HT LDG                                        GRN               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   112.4                  83  6.1                         9.0                            6.1                               68.9                                  28.5                                     96.3                                        4.0           2   123.6                  91  5.6                         9.0                            5.4                               74.6                                  33.1                                     92.5                                        4.7           LOCS               10 10  15 1  38 30 24 12 16           REPS               10 10  16 1  40 33 27 14 19           DIFF               11.2                  8   0.5                         0.0                            0.7                               5.6                                  4.6                                     3.7                                        0.7           PR > T               .471                  .512                      .108  .002#                               .000#                                  .000#                                     .370                                        .249    __________________________________________________________________________               STK                  BRT GRN                         SCT                            EAR                               TEX                                  EAR                                     BAR                                        DRP               LDG                  STK APP                         GRN                            SZ EAR                                  MLD                                     PLT                                        EAR               ABS                  ABS ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS    __________________________________________________________________________    TOTAL SUM           1   96.7                  100.0                      6.6                         7.6                            5.4                               3.7                                  5.9                                     97.7                                        99.5           2   92.9                  97.9                      6.2                         6.8                            6.0                               4.7                                  6.3                                     99.1                                        98.1           LOCS               12 8   7  10 9  9  12 19 9           REPS               16 8   10 11 9  10 13 19 11           DIFF               3.7                  2.1 0.4                         0.8                            0.6                               1.0                                  0.4                                     1.4                                        1.4           PR > T               .098*                  .019+                      .394                         .053*                            .179                               .003#                                  .269                                     .105                                        .269    __________________________________________________________________________               GLF                  NLF STW                         HD EYE                               COM                                  ECB                                     ECB               SPT                  BLT WLT                         SMT                            SPT                               RST                                  1LF                                     2SC               ABS                  ABS ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS    __________________________________________________________________________    TOTAL SUM           1   3.4                  4.8 3.0                         82.8                            7.0                               5.2                                  5.8                                     4.5           2   3.2                  7.5 5.0                         81.3                            5.0                               6.0                                  4.8                                     3.5           LOCS               5  4   3  1  1  3  8  2           REPS               5  4   3  2  1  5  8  2           DIFF               0.2                  2.8 2.0                         1.5                            2.0                               0.8                                  1.0                                     1.0           PR > T               .749                  .002#                      .074*    .199                                  .121                                     .000#    __________________________________________________________________________     * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 3A    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH0HR To PHP02 Crossed    To The Same Inbred Testers And Grown In The Same Experiments.    __________________________________________________________________________               SEL     BU  BU  PRM     TST SDG EST GDU               IND PRM ACR ACR SHD MST WT  VGR CNT SHD               % MN                   ABS ABS % MN                               ABS % MN                                       ABS % MN                                               % MN                                                   % MN    __________________________________________________________________________    TOTAL SUM           REPS               18  18  188 188 7   191 145 101 123 48           LOCS               18  18  170 170 7   172 129 89  108 43           PH0HR               98  105 151 104 104 102 55  106 102 100           PHP02               98  103 144 98  102 96  55  101 100 98           DIFF               0   2   7   5   1   6   0   5   2   2           PR > T               0.99                   0.00                       0.00                           0.00                               0.16                                   0.00                                       0.99                                           0.10                                               0.03                                                   0.00    __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN EYE               SLK CNT HT  HT  LDG GRN LDG STK APP SPT               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           % MN                                               % MN                                                   ABS    __________________________________________________________________________    TOTAL SUM           REPS               31  218 83  83  76  72  172 36  47  2           LOCS               27  193 80  80  67  65  157 30  42  2           PH0HR               99  101 96  99  97  113 100 104 100 6           PHP02               98  100 97  98  95  88  101 99  96  6           DIFF               2   0   1   1   3   25  1   5   4   0           PR > T               0.00                   0.99                       0.04                           0.21                               0.28                                   0.00                                       0.22                                           0.01                                               0.07                                                   0.99    __________________________________________________________________________               ECB ECB ECB DRP NLF               DPE 1LF 2SC EAR BLT               ABS ABS ABS % MN                               ABS    __________________________________________________________________________    TOTAL SUM           REPS               26  7   8   142 2           LOCS               25  7   8   133 1           PH0HR               99  3   4   99  6           PHP02               100 3   4   100 4           DIFF               0   0   1   1   2           PR > T               0.99                   0.99                       0.01                           0.53    __________________________________________________________________________     *PR > T values are valid only for comparisons with Locs > = 10.

                                      TABLE 3B    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH0HR To PH24E Crossed    To The Same Inbred Testers And Grown In The Same Experiments.    __________________________________________________________________________               SEL     BU  BU  PRM     TST SDG EST GDU               IND PRM ACR ACR SHD MST WT  VGR CNT SHD               % MN                   ABS ABS % MN                               ABS % MN                                       ABS % MN                                               % MN                                                   % MN    __________________________________________________________________________    TOTAL SUM           REPS               127 125 678 678 132 689 525 344 455 216           LOCS               127 125 666 666 132 677 514 333 443 215           PH0HR               97  107 150 101 104 103 55  104 100 100           PH24E               102 105 153 102 104 101 54  95  99  100           DIFF               5   1   3   2   0   3   0   9   1   0           PR > T               0.00                   0.00                       0.00                           0.00                               0.99                                   0.00                                       0.99                                           0.00                                               0.02                                                   0.99    __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN EYE               SLK CNT HT  HT  LDG GRN LDG STK APP SPT               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           % MN                                               % MN                                                   ABS    __________________________________________________________________________    TOTAL SUM           REPS               158 785 327 327 261 255 608 121 160 4           LOCS               158 759 327 327 260 245 598 121 149 4           PH0HR               100 100 97  98  101 112 100 107 101 5           PH24E               100 99  98  99  99  105 101 100 100 5           DIFF               0   1   2   1   2   7   0   7   0   0           PR > T               0.99                   0.00                       0.00                           0.01                               0.06                                   0.00                                       0.99                                           0.00                                               0.99                                                   0.99    __________________________________________________________________________               ECB ECB ECB DRP GLF NLF               DPE 1LF 2SC EAR SPT BLT               ABS ABS ABS % MN                               ABS ABS    __________________________________________________________________________    TOTAL SUM           REPS               123 38  22  526 1   19           LOCS               123 38  22  516 1   10           PH0HR               100 3   5   100 4   7           PH24E               100 3   4   100 4   6           DIFF               0   0   1   0   0   0           PR > T               0.99                   0.99                       0.00                           0.99                               0.99                                   0.99    __________________________________________________________________________     *PR > T values are valid only for comparisons with Locs > = 10.

                                      TABLE 4A    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PHP02/PHRD6    __________________________________________________________________________                   PRM BU  BU      TST SDG EST GDU               PRM SHD ACR ACR MST WT  VGR CNT SHD               ABS ABS ABS % MN                               % MN                                   ABS % MN                                           % MN                                               % MN    __________________________________________________________________________    TOTAL SUM           1   100 98  143.4                           103 100 54.9                                       112 101 97           2   99  96  139.8                           100 99  55.8                                       87  104 96           LOCS               12  14  42  42  42  32  42  54  18           REPS               12  14  59  59  60  47  61  73  25           DIFF               0   2   3.6 3   1   0.9 25  3   1           PR > T               .999                   .000#                       .148                           .138                               .217                                   .000#                                       .000#                                           .054*                                               .002#    __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN               SLK CNT HT  HT  LDG GRN LDG STK APP               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           % MN                                               % MN    __________________________________________________________________________    TOTAL SUM           1   97  100 94  99  95  106 97  108 100           2   95  101 95  91  99  97  96  106 104           LOCS               11  88  27  27  14  17  37  19  10           REPS               17  134 38  38  23  24  51  29  13           DIFF               2   1   2   8   3   9   1   2   4           PR > T               .000#                   .123                       .051*                           .000#                               .485                                   .315                                       .645                                           .229                                               .052*    __________________________________________________________________________               DRP ECB ECB ECB               EAR DPE 1LF 2SC               % MN                   ABS ABS ABS    __________________________________________________________________________    TOTAL SUM           1   100 99.1                       3.0 7.0           2   100 99.2                       3.0 6.0           LOCS               27  10  1   1           REPS               36  11  1   1           DIFF               0   0.1 0.0 1.0           PR > T               .999                   .830    __________________________________________________________________________     * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 4B    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PHRE1/PHP02    __________________________________________________________________________                   PRM BU  BU      TST SDG EST GDU               PRM SHD ACR ACR MST WT  VGR CNT SHD               ABS ABS ABS % MN                               % MN                                   ABS % MN                                           % MN                                               % MN    __________________________________________________________________________    TOTAL SUM           1   100 98  155.4                           105 99  55.1                                       111 103 98           2   97  95  142.2                           96  92  55.0                                       106 103 95           LOCS               25  26  82  82  85  54  64  74  39           REPS               25  26  102 102 106 70  80  91  48           DIFF               3   3   13.3                           9   7   0.1 5   1   3           PR > T               .000#                   .000#                       .000#                           .000#                               .000#                                   .726                                       .148                                           .413                                               .000#    __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN               SLK CNT HT  HT  LDG GRN LDG STK APP               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           % MN                                               % MN    __________________________________________________________________________    TOTAL SUM           1   97  101 95  100 95  107 99  108 100           2   95  102 95  97  92  74  100 96  97           LOCS               26  144 44  44  33  35  78  19  16           REPS               33  196 51  51  44  43  95  30  21           DIFF               3   1   0   3   4   33  1   12  3           PR > T               .000#                   .072*                       .999                           .004#                               .423                                   .000#                                       .435                                           .010+                                               .275    __________________________________________________________________________               DRP NLF GIB EYE ECB ECB ECB               EAR BLT ERS SPT DPE 1LF 2SC               % MN                   ABS ABS ABS ABS ABS ABS    __________________________________________________________________________    TOTAL SUM           1   98  5.5 3.0 6.0 98.9                                   3.0 3.2           2   99  3.5 6.0 5.0 99.0                                   4.0 2.5           LOCS               62  1   1   3   13  1   6           REPS               71  2   1   3   14  1   6           DIFF               1   2.0 3.0 1.0 0.2 1.0 0.7           PR > T               .428        .423                               .805    .025+    __________________________________________________________________________     * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 4C    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PHP02/PHTM9    __________________________________________________________________________               PRM BU  BU      TST SDG EST GDU GDU STK           PRM SHD ACR ACR MST WT  VGR CNT SHD SLK CNT           ABS ABS ABS % MN                           % MN                               ABS % MN                                       % MN                                           % MN                                               % MN                                                   % MN    __________________________________________________________________________    TOTAL SUM    1      100 98  148.9                       104 100 54.7                                   112 101 97  97  100    2      94  97  138.3                       97  88  55.4                                   106 103 96  96  102    LOCS   15  14  42  42  44  30  47  58  20  15  97    REPS   15  14  52  52  55  38  59  67  23  18  131    DIFF   6   1   10.5                       8   12  0.7 6   2   1   1   2    PR > T .000#               .103                   .000#                       001#                           .000#                               .003#                                   .166                                       .122                                           .044+                                               .372                                                   .007#    __________________________________________________________________________           PLT EAR RT  STA STK BRT GRN DRP ECB ECB ECB           HT  HT  LDG GRN LDG STK APP EAR DPE 1LF 2SC           % MN               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           ABS ABS ABS    __________________________________________________________________________    TOTAL SUM    1      94  98  97  104 100 105 111 100 98.9                                               3.0 7.0    2      101 98  99  55  97  104 103 100 99.1                                               4.0 7.0    LOCS   24  24  21  16  39  10  2   32  8   1   1    REPS   32  32  30  21  47  16  2   38  8   1   1    DIFF   6   1   2   48  3   1   8   0   0.2 1.0 0.0    PR > T .000#               .629                   .590                       .000#                           .291                               .860                                   .500                                       .999                                           .816    __________________________________________________________________________     *= 10% SIG     += 5% SIG     #= 1% SIG

                                      TABLE 4D    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PHRE1/PHTD5    __________________________________________________________________________              PRM                 BU BU      TST                               SDG EST GDU GDU           PRM              SHD                 ACR                    ACR MST WT VGR CNT SHD SLK           ABS              ABS                 ABS                    % MN                        % MN                            ABS                               % MN                                   % MN                                       % MN                                           % MN    __________________________________________________________________________    TOTAL SUM    1      100              97 149.2                    114 106 54.8                               103 101 99  98    2      89 90 114.3                    86  89  57.5                               104 98  92  92    LOCS   5  5  10 10  11  7  24  20  7   7    REPS   5  5  17 17  20  13 29  25  10  10    DIFF   11 7  34.9                    28  17  2.8                               0   2   6   7    PR > T .000#              .001#                 .000#                    .000#                        .000#                            .019+                               .999                                   .380                                       .000#                                           .000#    __________________________________________________________________________           STK PLT EAR RT  STA STK BRT DRP ECB           CNT HT  HT  LDG GRN LDG STK EAR 1LF           % MN               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           ABS    __________________________________________________________________________    TOTAL SUM    1      103 97  96  101 120 103 103 100 3.0    2      98  90  94  100 79  100 111 100 5.0    LOCS   36  7   7   6   4   9   5   9   1    REPS   52  10  10  12  7   15  9   15  1    DIFF   5   7   3   1   41  3   7   0   2.0    PR > T .191               .000#                   .543                       .612                           .023+                               .511                                   .321                                       .999    __________________________________________________________________________     *= 10% SIG     += 5% SIG     #= 1% SIG

                                      TABLE 4E    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PHP02/PHOAV    __________________________________________________________________________              PRM                 BU BU      TST                               SDG EST GDU GDU STK PLT           PRM              SHD                 ACR                    ACR MST WT VGR CNT SHD SLK CNT HT           ABS              ABS                 ABS                    % MN                        % MN                            ABS                               % MN                                   % MN                                       % MN                                           % MN                                               % MN                                                   % MN    __________________________________________________________________________    TOTAL SUM    1      100              98 146.7                    106 101 55.0                               110 101 98  98  100 95    2      97 98 134.9                    97  94  55.3                               98  102 98  97  101 99    LOCS   14 14 37 37  37  21 50  51  22  18  93  21    REPS   14 14 49 49  50  29 57  58  27  23  117 30    DIFF   3  0  11.9                    9   7   0.3                               12  0   0   0   2   4    PR > T .010+              .999                 .000#                    .000#                        .000#                            .305                               .007#                                   .999                                       .999                                           .999                                               .059*                                                   .000#    __________________________________________________________________________           EAR RT  STA STK BRT GRN DRP GLF GIB ECB ECB           HT  LDG GRN LDG STK APP EAR SPT ERS DPE 1LF           % MN               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       ABS ABS ABS ABS    __________________________________________________________________________    TOTAL SUM    1      100 96  118 101 110 103 96  4.5 3.0 98.7                                                   3.0    2      102 107 91  96  95  100 99  5.5 7.0 98.5                                                   7.0    LOCS   21  16  17  32  11  2   28  1   1   4   i    REPS   30  23  23  41  16  3   34  2   1   4   1    DIFF   2   11  27  4   15  3   3   1.0 4.0 0.2 4.0    PR > T .259               .448                   .000#                       .057*                           .092*                               .815                                   .387        .819    __________________________________________________________________________     *= 10% SIG     += 5% SIG     #= 1% SIG

                                      TABLE 4F    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PH0HC/PH24E    __________________________________________________________________________              PRM                 BU BU      TST                               SDG EST GDU GDU STK PLT EAR           PRM              SHD                 ACR                    ACR MST WT VGR CNT SHD SLK CNT HT  HT           ABS              ABS                 ABS                    % MN                        % MN                            ABS                               % MN                                   % MN                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN    __________________________________________________________________________    TOTAL SUM    1      100              98 145.9                    102 97  55.1                               112 101 97  97  100 93  99    2      103              100                 151.8                    105 103 55.0                               112 98  98  98  98  99  104    LOCS   15 20 65 65  66  36 58  57  33  20  120 37  37    REPS   15 20 81 81  82  43 74  69  42  24  164 52  52    DIFF   2  1  5.9                    4   6   0.1                               1   4   1   2   3   6   4    PR > T .000#              .004#                 .007#                    .021+                        000#                            .530                               .842                                   .005#                                       .003#                                           .002#                                               .001#                                                   .000#                                                       .002#    __________________________________________________________________________           RT  STA STK BRT GRN DRP GLF                                      NLF                                         GOS                                            ANT                                               HD GIB                                                     ECB                                                        ECB           LDG GRN LDG STK APP EAR SPT                                      BLT                                         WLT                                            ROT                                               SMT                                                  ERS                                                     DPE                                                        2SC           % MN               % MN                   % MN                       % MN                           % MN                               % MN                                   ABS                                      ABS                                         ABS                                            ABS                                               ABS                                                  ABS                                                     ABS                                                        ABS    __________________________________________________________________________    TOTAL SUM    1      95  101 99  104 98  97  4.0                                      5.5                                         7.8                                            1.0                                               95.7                                                  2.8                                                     90.7                                                        3.7    2      110 112 102 91  99  100 4.1                                      5.6                                         8.0                                            1.0                                               85.1                                                  4.0                                                     91.3                                                        3.7    LOCS   26  32  58  15  10  44  4  4  2  1  1  2  17 7    REPS   36  43  73  22  14  50  8  8  3  2  2  3  23 7    DIFF   15  12  3   13  1   3   0.1                                      0.1                                         0.3                                            0.0                                               10.7                                                  1.3                                                     0.5                                                        0.0    PR > T .133               .030+                   .056                       .139                           .719                               .210                                   .878                                      .824                                         .500     .344                                                     .693                                                        .999    __________________________________________________________________________     *= 10% SIG     += 5% SIG     #= 1% SIG

                                      TABLE 4G    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PH0HC/PH24E    __________________________________________________________________________                   PRM BU  BU      TST SDG EST GDU GDU STK PLT EAR               PRM SHD ACR ACR MST WT  VGR CNT SHD SLK CNT HT  HT               ABS ABS ABS % MN                               % MN                                   ABS % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           1   100 98  137.6                           100 94  55.9                                       119 101 95  95  101 93  98           2   104 106 147.9                           107 102 55.2                                       91  100 101 101 100 99  100           LOCS               8   10  30  30  31  19  29  38  19  12  74  17  17           REPS               8   10  34  34  35  23  34  47  21  13  99  19  19           DIFF               2   8   10.4                           8   8   0.7 28  1   7   6   1   6   1           PR > T               .001#                   .000#                       .003#                           .004+                               000#                                   .071*                                       .000#                                           .534                                               .000#                                                   .000#                                                       .412                                                           .000#                                                               .555    __________________________________________________________________________               RT  STA STK BRT DRP GFL NFL GOS ANT HD  GIB ECB ECB               LDG GRN LDG STK EAR SPT BLT WLT ROT SMT ERS DPE 2SC               % MN                   % MN                       % MN                           % MN                               % MN                                   ABS ABS ABS ABS ABS ABS ABS ABS    __________________________________________________________________________    TOTAL SUM           1   95  73  99  107 99  4.0 5.5 7.8 1.0 95.7                                                       2.8 98.1                                                               3.2           2   103 101 103 104 100 3.8 6.4 7.0 1.0 80.3                                                       2.5 99.1                                                               2.7           LOCS               9   5   26  10  19  4   4   2   1   1   2   8   6           REPS               9   5   29  18  19  8   8   3   2   2   3   8   6           DIFF               8   28  5   3   1   0.3 0.9 0.8 0.0 15.4                                                       0.3 1.0 0.5           PR > T               .457                   .521                       .237                           .232                               .096*                                   .604                                       .293                                           .656        .500                                                           .280                                                               .203    __________________________________________________________________________     *= 10% SIG     += 5% SIG     #= 1% SIG

                                      TABLE 4H    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PHRE1/PH0HR    VARIETY #2 = PH44A/PH24E    __________________________________________________________________________               PRM BU  BU      TST SDG EST GDU GDU STK           PRM SHD ACR ACR MST WT  VGR CNT SHD SLK CNT           ABS ABS ABS % MN                           % MN                               ABS % MN                                       % MN                                           % MN                                               % MN                                                   % MN    __________________________________________________________________________    TOTAL SUM    1      101 98  135.2                       100 92  55.4                                   113 102 94  94  101    2      105 107 145.3                       107 102 54.6                                   87  95  101 100 97    LOCS   4   7   16  16  17  10  8   4   12  7   26    REPS   4   7   16  16  17  10  9   4   13  7   34    DIFF   4   8   10.1                       7   10  0.8 25  6   6   6   4    PR > T .002#               .000#                   .036+                       .087*                           .000#                               .153                                   .085*                                       .264                                           .000#                                               .000#                                                   .064*    __________________________________________________________________________           PLT EAR RT  STA STK BRT DRP GLF GIB ECB ECB           HT  HT  LDG GRN LDG STK EAR SPT ERS DPE 2SC           % MN               % MN                   % MN                       % MN                           % MN                               % MN                                   % MN                                       ABS ABS ABS ABS    __________________________________________________________________________    TOTAL SUM    1      92  97  102 73  101 111 100 4.5 3.0 98.0                                                   3.2    2      100 100 97  109 103 104 110 5.0 3.0 99.1                                                   3.0    LOCS   8   8   8   5   15  5   15  1   1   5   6    REPS   9   9   8   5   15  7   15  2   1   5   6    DIFF   8   3   5   36  2   7   1   0.5 0.0 1.1 0.2    PR > T .003#               .333                   .330                       .188                           .467                               .393                                   .059*       .451                                                   .741    __________________________________________________________________________     *= 10% SIG     += 5% SIG     #= 1% SIG

Deposits

Applicant has made a deposit of at least 2500 seeds of Inbred Maize LinePH0HR with the American Type Culture Collection (ATCC), Manassas, Va.20110 USA, ATCC Deposit No. 209943. The seeds deposited with the ATCC onJun. 4, 1998 were taken from the deposit maintained by Pioneer Hi-BredInternational, Inc., 700 Capital Square, 400 Locust Street, Des Moines,Iowa 50309-2340 since prior to the filing date of this application. Thisdeposit of the Inbred Maize Line PH0HR will be maintained in the ATCCdepository, which is a public depository, for a period of 30 years, or 5years after the most recent request, or for the effective life of thepatent, whichever is longer, and will be replaced if it becomesnonviable during that period. Additionally, Applicant has satisfied allthe requirements of 37 C.F.R. §§1.801-1.809, including providing anindication of the viability of the sample. Applicant imposes norestrictions on the availability of the deposited material from theATCC; however, Applicant has no authority to waive any restrictionsimposed by law on the transfer of biological material or itstransportation in commerce. Applicant does not waive any infringement ofits rights granted under this patent or under the Plant VarietyProtection Act (7 USC 2321 et seq.). Applicant has applied for U.S.Plant Variety Protection of PH0HR under Application No. 9700205.

The foregoing invention has been described in detail by way ofillustration and example for purposes of clarity and understanding.However, it will be obvious that certain changes and modifications suchas single gene modifications and mutations, somoclonal variants, variantindividuals selected from large populations of the plants of the instantinbred and the like may be practiced within the scope of the invention,as limited only by the scope of the appended claims.

What is claimed is:
 1. Seed of maize inbred line designated PH0HR,representative samples having been deposited under ATCC Accession No.209943.
 2. A maize plant, or parts thereof, having all the physiologicaland morphological characteristics of inbred line PH0HR, representativeseed of said line having been deposited under ATCC Accession No. 209943.3. The maize plant of claim 2, wherein said plant is male sterile.
 4. Atissue culture of regenerable cells of a maize plant of inbred linePH0HR, wherein the tissue regenerates plants capable of expressing allthe morphological and physiological characteristics of the inbred linePH0HR, representative seed of which have been deposited under ATCCAccession No.
 209943. 5. A tissue culture according to claim 4, thecells or protoplasts being from a tissue selected from the groupconsisting of leaves, pollen, embryos, roots, root tips, anthers, silks,flowers, kernels, ears, cobs, husks, and stalks.
 6. A maize plantregenerated from the tissue culture of claim 4, said plant, capable ofexpressing all the morphological and physiological characteristics ofinbred line PH0HR, representative seed of which have been depositedunder ATCC Accession No.
 209943. 7. A method for producing a firstgeneration (F₁) hybrid maize seed comprising crossing the plant of claim2 with a different inbred parent maize plant and harvesting theresultant first generation (F₁) hybrid maize seed.
 8. The method ofclaim 7 wherein inbred maize plant of claim 2 is the female or maleparent.
 9. An F₁ hybrid seed produced by crossing the inbred maize plantaccording to claim 2 with another, different maize plant.
 10. An F₁hybrid plant, or parts thereof, grown from the seed of claim 9.